Fig. 8

Mouse single-cell transcriptomic analysis revealed distinct immune and stromal features of immune-related myocarditis. (A) UMAP projection of cardiac cell populations in murine hearts across four groups: control, irAE, viral, and autoimmune myocarditis. (B) Group prevalence of each T/NK cell subset estimated by the Ro/e score, representing the ratio of observed to expected cell numbers. (C) Boxplots showing the exhaustion and glycolysis signature scores of all T/NK cells in each group. P values were determined by the Wilcoxon test. (D) Heatmap showing the M1, M2, pro-inflammatory, and anti-inflammatory signature scores of monocyte/macrophage subpopulations across four groups. (E) Bubble plot showing the enriched ligand-receptor pairs of TNF and CCL signaling pathways between monocytes/macrophages and endothelial cells. (F) Group prevalence of each fibroblast subset estimated by the Ro/e score. (G) Heatmap of normalized PROGENy pathway activity scores of fibroblast subpopulations in murine hearts. (H) Incoming and outgoing interaction strengths of all cell subpopulations in murine heart tissues. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 were considered statistically significant. irAE, immune-related adverse event; UMAP, Uniform Manifold Approximation and Projection; TNF, tumor necrosis factor; CCL, C-C motif chemokine ligand