Fig. 4
Increased cell-cell communications among immune cells in the irAE group. (A) Bar plots showing the number of interactions and interaction strength among immune cells in the irAE and normal groups. (B) Circle plot showing differential interaction strength among immune cell subpopulations between the irAE group and the normal group. Red colored lines represent increased signaling and blue colored lines represent decreased signaling in the irAE group. (C) Heatmap illustrating differential interaction strength among immune cell subsets in the irAE group vs. the normal group. The x-axis represents the signaling receivers and the y-axis represents the signaling senders. (D) Circle plots showing the CCL signaling pathway networks among immune cells in the irAE and normal groups. (E) Circle plots showing the TNF signaling pathway networks among immune cells in the irAE and normal groups. (F) Bubble plot showing the enriched ligand-receptor pairs among immune cells with color representing the communication probability and size reflecting the p-value significance. CD8 + Tem, CD8 + effector memory T; CD8 + Tex, CD8 + exhausted T; CD8 + Tprolif, CD8 + proliferative T; NK, natural killer; Antigen-presenting Macro, antigen-presenting macrophage; CD14 + Mono, CD14 + monocyte; CD16 + Mono, CD16 + monocyte; LYVE1 + Macro, LYVE1 + macrophage; CCL, C-C motif chemokine ligand; TNF, tumor necrosis factor; irAE, immune-related adverse event