Fig. 1

Single-cell landscape of immune and stromal cells of heart tissues. (A) Workflow of the overall study design. Created with BioRender.com. (B) Hematoxylin-eosin (H&E) staining and immunohistochemistry (IHC) staining (positive for CD3, CD4, CD8, and CD63; negative for CD20) of endomyocardial biopsies from the patient diagnosed with immune-related myocarditis (150× magnification). (C) Uniform Manifold Approximation and Projection (UMAP) projection of all captured cells which are colored by major cell types. (D) Dot plot showing the expression of marker genes of seven major cell types. The dot size and color indicate the percentage and level of expression, respectively. (E) Heatmap of the top 5 differentially expressed genes (DEGs) expression of each major cell cluster. (F) Cell type correlation heatmap showing the relationship among seven major cell subpopulations based on the average expression of the top 2,000 variable genes ranked by standard deviation, using Spearman’s correlation analysis. (G) Proportions of major cell types in the irAE and normal groups. irAE, immune-related adverse event