Fig. 2

CLSPN promotes the proliferation, migration of melanoma cells. (A-B) The mRNA and protein expression levels of CLSPN in A375, A2058 cell lines after si-CLSPN transfection. (C) Cell viability of A375 and A2058 after knockdown of CLSPN. (D) Colony formation analysis of A375 and A2058 after knockdown of CLSPN. (E) Representative images of migration in A375 and A2058. Cells were pretreated with 20 µM zVAD for 2 h and then subjected to CLSPN knockdown. Data were expressed as mean ± SD. * p < 0.05, ** p < 0.01 and *** p < 0.001