Fig. 2
The anti-tumor effect of F1929-1458 is mediated by enhancing T cell infiltration. A–C FACS analysis of CD3+ T cells (B) and CD3+CD8+ T cells (C) in CT26 graft tumors treated with control (ddH2O), 5 mg/kg and 15 mg/kg doses of F1929-1458 and positive control (5-FU). D Immunofluorescence staining and quantification of CD3+ T cells in CT26 graft tumors treated with control (ddH2O) or F1929-1458 (n = 5). Scale bars, 25 μm. E The frequency of CD3 + T cells in peripheral blood mononuclear cells (PBMCs) from both treated and untreated mice. F The image of CT26 graft tumors in nude mice treated with control (ddH2O) or F1929-1458 (n = 8). The relative tumor volumes (G). The relative tumor weights (H). I Neutralization of T cells in wild-type BALB/c mice by using an anti-CD3 antibody. J, K The tumor volumes (J) and the relative tumor weights (K) of CT26 graft tumors with indicated treatments