Fig. 2

Effects of Fn on the alveolar bone and osteogenesis differentiation. (A) ELISA of TNF-a, IL-6, IL-1β and IL-8 in serum from mice experimental periodontitis model (n = 3). (B) Micro-CT reconstruction of alveolar bone and tooth, and quantitative analyses of CEJ-ABC (n = 7). (C) Representative images of H&E staining of alveolar bone (n = 7). Scale bars, 200 μm. (D) Representative images of Col1 staining and quantitative analysis (n = 7). (E) Representative images of TRAP staining of alveolar bone and quantitative analysis (n = 7). (F) Statistical results of the cell proliferation ratio of osteoblasts detected by EdU assay. (G-H) Statistical analysis of cell apoptosis ratio of osteoblasts analyzed by flow cytometry (n = 3). (I) Representative images of ALP staining in osteoblasts cocultured with Fn (n = 3). (J) Representative images of alizarin red staining in osteoblasts cocultured with Fn at 21 days (n = 3). (K) Calcium quantification from samples in (J). (L) Relative mRNA expression of Col1, Runx2 and Bmp2 in osteoblasts after Fn infection (n = 3). (M) Protein expression of Col1, Runx2 and Bmp2 in osteoblasts after Fn infection at days 7 and 14 (n = 3). Data are presented as the mean ± SD. One-way ANOVA (F-H) and Student’s t test (A, B, D, E, K) were used to examine the statistical significance between groups, *P < 0.05, **P < 0.01, and ***P < 0.001