Fig. 7

RNASET2 expression is down-regulated in monocytes and macrophages derived from prostate cancer samples compared with normal tissues. TISCH2 analysis of RNASET2 expression among the tumor microenvironment components of prostate cancer in 6 different datasets (GSE141445, GSE143791, GSE137829, GSE150692, GSE172301, and GSE176031) (A). GSE153892 [36] dataset showing CD45⁺ cells sorted from human prostatic tumoral (n = 3) and non-tumoral tissues (n = 3) was re-analyzed. UMAP-based 2D visualization describing cell clusters and distinct cell types represented by different color codes are shown together with overall RNASET2 expression (B). Volcano plot displays the results of differentially expression analysis and shows the up-regulated and down-regulated genes in prostate cancer patients’ tissues (PC) and non-tumoral tissues (HC) (C). To assess the different expression of RNASET2, samples were divided in non-tumoral tissues (HC) and prostate cancer tumoral tissues (PC) (D). Increased expression of RNASET2 in monocytes from healthy control subjects compared to monocytes from PCa patients, a detected in our small cohort of subjects, by flow cytometry (E). Correlation plot generated using the TIMER2 software to interrogate the relation between RNASET2 expression and M1 or M2 macrophage infiltration in PCa tumor tissues (F)