Fig. 6

Effects of RNASET2 on tumor cell growth in vivo. The effects of RNASET2-overxpression in PCa cells were investigated on tumor cell growth in vivo. Nude Mice-Nu/Nu male mice (n = 5) were subcutaneously injected with RNASET2-overexpressing. Growth curve graphs (A, D), photographs of excised tumors (B, E), and tumor weight (C, F) of RNASET2-overexpressin PC-3 and 22Rv1 PCa tumor xenografts, and their related empty-vector transfected controls, are shown. FACS analysis (n = 3 animals/group) for tumor-infiltrating CD80⁺ M1 and CD206⁺ M2 macrophages (G, J), M1/M2 ratio (H, K), and representative dot plots (I, L) in excised RNASET2-overexpressing PC-3 and 22Rv1 PCa xenograft, and their related empty-vector transfected controls. Data are showed as mean ± SEM, Two-Way ANOVA (for growth curves) and One-Way ANOVA (for flow cytometry results)