Fig. 5

Secretome analysis of soluble factors released by RNASET2-empty and RNASET2-overexpressing PC-3 and 22Rv1 cells. Heatmap showing the modulation of the main immunomodulatory factors involved in monocyte/macrophage recruitment and polarization in the conditioned media from RNASET2-empty vector and RNASET2-overexpressing PC-3 cells (A) and RNASET2-empty vector and RNASET2-overexpressing 22Rv1 cells (B). Bar plot showing the modulation of the selected soluble factors by PC-3 and 22Rv1 cells overexpressing RNASET2 represented as fold change over the respective empty controls (C). String analysis for the biological connections among the most upregulate soluble factors, in RNASET2-expressing PCa cells, as detected by secretome analysis of their related conditioned media (D). Transmission electron microscopy (TEM) of PC-3 and 22Rv1 RNASET2 overexpressing and empty control PCa cells, and related graphs showing the quantification of Reticulum (R) and Golgi (G) hypertrophy. Data are shown as mean ± SEM, One-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001, or mean ± SEM student t-test *p < 0.05