Fig. 3

Effects of RNASET2 on cell adhesion migration and cytoskeleton modification in PCa cell lines. The effect or RNASET2 expression on key metastatic features of PCa cells was investigated: adhesion assay (A, B) (n = 3, in triplicate), migration assay (n = 2, in triplicate), in presence or absence of FBS as driving stimulus (C, D) and cytoskeleton morphological analysis (n = 4), with white arrowheads pointing at cell spreading processes observed in PC-3 cells and actin clustering at the cell periphery in 22Rv1 cells, respectively (E, F). RNASET2 (green) and Phalloidin (red) staining are shown. Data are showed as mean ± SEM, t-student test, *p < 0.05; **p < 0.01. E: empty control cells; T2: RNASET2-overexpressing cells