Fig. 2

Loss of Rasal2 promotes autophagic-exos secretion in TNBC. a, Construction of Rasal2 KO MDA-MB-231 cell lines by CRISPR/Cas9 technique. b, The protein expression of Rasal2 detected by western blot analysis. c, The effect of Rasal2 KO on exosomal secretion analyzed by NTA. d-e, Effects of Rasal2 KO on the expression levels of autophagic and exosomal marker proteins in exos. f, Establishment of CT and KO MDA-MB-231 cell lines with GFP-LC3 stable overexpression. g, Fluorescence intensity of magnetic beads-bound exos derived from CT and KO MDA-MB-231 cells stably overexpressing GFP-LC3