Fig. 3
Exploratory efficacy analysis and characterization of hNSC engraftment following bilateral ICV transplantation under high-dose prolonged immunosuppression. a) Schematic showing the experimental setup of a high-dose hNSC bilateral ICV transplantation under high-dose prolonged immunosuppression (EXP 5) in n = 13 SOD1G93A mice. b - c) Endurance loss visualization of motor performance decline (b) and weight measurements (c) of hNSC- (n = 13, blue line) vs. HBSS-treated SOD1G93A mice (n = 11, red line); Two-way ANOVA, uncorrected Fisher’s LSD test, Mixed-effect model (p-value: *≤0.05). d) Pie chart showing the percentage of animals retrieved with viable hNSC grafts over the total treated animals (n = 13), and representative confocal images of huN+ engrafted hNSCs at 40 dpt.| huN, green; GFAP, red; DAPI, blue. e) Quantification of the absolute number of engrafted hNSCs in the entire brain of SOD1G93A mice (n = 6) at 40 dpt. f) Pie chart and representative confocal images showing the relative percentages of non-apoptotic (huN+/TUNEL−) and apoptotic (huN+/TUNEL+) engrafted hNSCs over the total huN+ cells in n = 3 SOD1G93A mice at 40 dpt.| huN, green; TUNEL, red; DAPI, blue. All data are expressed as mean values ± SEM. Drawings on this figure were created with BioRender