Fig. 2
hNSC survival probability assessment and exploratory efficacy analysis of a bilateral ICV transplantation of high-dose hNSCs under high-dose transient immunosuppression. (a) Schematic showing the experimental setup of a bilateral high-dose (1 × 106) hNSC ICV transplantation under high-dose (30 mg/kg daily) transient (15 days) immunosuppression regimen in SOD1G93A mice from EXP 3 and 4 as well as n = 2 at both 40 dpt and ES. (b) Endurance loss visualization of motor performance decline considering a total of n = 6 SOD1G93A mice + hNSCs (blue line) vs. n = 6 SOD1G93A mice + HBSS (red line) (EXP 3); Two-way ANOVA, uncorrected Fisher’s LSD test, Mixed-effect model. (c) Representative confocal images showing the time-course assessment of post-transplantation hNSCs viability and differentiation profile under a high-dose transient immunosuppression regimen at 7 (n = 2), 15 (n = 2), and 40 (n = 6) dpt.| huN, green; GFAP, red; DAPI, blue. (d) Neuropathological analysis of the M1-V layer’s astrogliosis at both 40 dpt and ES on hNSC- vs. HBSS-treated mice; n ≥ 3/time point/group (EXP 3 and 4); parametric unpaired t-test with Welch’s correction| GFAP, red; DAPI, blue. (e) Neuropathological analysis of the M1-V layer’s %Area of IBA1+ signal at both 40 dpt and ES on hNSC- vs. HBSS-treated mice; n ≥ 3/time point/group (EXP 3 and 4); parametric unpaired t-test with Welch’s correction| IBA1, green; DAPI, blue. (f) Neuropathological analysis of the M1-V layer’s assessed counting the total number of microglial cells per mm2 on hNSC- vs. HBSS-treated mice at both 40 dpt and ES; n ≥ 3/time point/group (EXP 3 and 4); nonparametric unpaired t-test with Mann-Whitney’s correction and parametric unpaired t-test with Welch’s correction for mice at 40 dpt and ES, respectively| IBA1, green; DAPI, blue. (g) Upper MN stereological count in hNSC- vs. HBSS-treated mice at 40 dpt and ES; n ≥ 3/time point/group (EXP 3 and 4); parametric unpaired t-test with Welch’s correction. All data are expressed as mean values ± SEM. Drawings on this figure were created with BioRender