Fig. 3

Selinexor and JQ1 regulated the expression of C-MYC. (A-B) Expression levels of C-MYC at different concentrations of Selinexor and JQ1 in AML cells, respectively in cells MV4-11 and THP-1 at 24 h (A) and 48 h (B). (C) Detect the IC50 concentration of Selinexor in MV4-11 cells with scramble or BRD4-SgRNA. (D) Cell viability of MV4-11 cells with scramble or BRD4-SgRNA treated with different doses of Selinexor. (E) Expression levels of BRD4 and C-MYC proteins in MV4-11 cells with scramble or BRD4-SgRNA. (F) Detect the IC50 concentration of Selinexor in cells THP-1 cells with scramble or BRD4-SgRNA. (G) Cell viability of THP-1 cells with scramble or BRD4-SgRNA treated with different doses of Selinexor. (H) Expression levels of BRD4 and C-MYC proteins in THP-1 cells with scramble or BRD4-SgRNA. (I) Protein expression levels of C-MYC in MOLM13 cells transfected with vector or overexpressed C-MYC plasmid. (J) Cell viability of MOLM13 cells with scramble or C-MYC treated with different doses of Selinexor and JQ1. (K-L) RNA expression of C-MYC in MV4-11 (K) and MOLM13 (L) cells treated with different doses of Selinexor(50nM) and JQ1(50nM). All experiments were performed with at least three independent replicates