Fig. 6

Cellular landscape of HCC from scRNA-seq analysis. (A) UMAP plot illustrating batch-corrected integration of 13 scRNA-seq samples (GSE149614), comprising 10 HCC patients, using the Harmony algorithm. B Clustering of 71,915 high-quality cells into 17 distinct clusters using the Seurat R package (resolution = 0.4). C Annotation of cell types based on canonical markers: T/NK cells, B cells, plasma cells, malignant cells, macrophages, monocytes, fibroblasts, endothelial cells, pericytes, and cycling cells. D Dot plot showing expression patterns of classical marker genes used to annotate cell types in different clusters. E UMAP plot visualizing expression patterns of key marker genes