Fig. 1

PARPi-resistant ovarian cancer cells obtain increased stemness. A Clonogenic assay of A2780 and A2780-olaR cells with DMSO or olaparib. B Cell viability curve of A2780 and A2780-olaR cells exposed to different concentration of olaparib for 72 h. C Clonogenic assay of SKOV3 and SKOV3-olaR cells with DMSO or olaparib. D Cell viability curve of SKOV3 and SKOV3-olaR cells exposed to different concentration of olaparib for 72 h. E Representative pictures and statistical results of A2780 and A2780-olaR cell spheroidization. F Flow chart and statistical diagram of ALDH-positive cells in A2780 and A2780-olaR cells. G Representative pictures and statistical results of SKOV3 and SKOV3-olaR cell spheroidization. H Flow chart and statistical diagram of ALDH-positive cells in SKOV3 and SKOV3-olaR cells. I GO analysis of RNA expression of A2780-olaR compared to A2780 based on GSE153867 data set. J Western blot image of SOX2, KLF4, Nanog and OCT4 protein expression in A2780, A2780-olaR, SKOV3 and SKOV3-olaR cells. P value was obtained by Student’s t-test. Results represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001