Fig. 7
From: ESPN activates ZEB1-mediated EMT through the PI3K/AKT/mTOR axis to promote osteosarcoma metastasis
ESPN promotes OS metastasis in vivo (A) A lung metastasis model was established in nude mice by injecting 143B cells from the control group (sh-CON), the ESPN knockdown group (sh-1), and the ESPN knockdown with ZEB1 overexpression group (sh-1 + ZEB1). Tail vein (n = 5/group). Top: Representative lung images. Bottom: Representative HE staining results. Pulmonary nodules are indicated by black triangles. Scale bar = 5 mm. (B) The number of metastatic pulmonary nodules in the specified group. (C) MG63 cells from different treatment groups (Vector, ESPN and ESPN + sh-ZEB1) were injected into the tail vein of nude mice to establish a lung metastasis model (n = 5/group). Top: Representative lung images; Bottom: Representative HE staining results. Pulmonary nodules are indicated by black triangles. Scale bar = 5 mm. (D) The number of metastatic pulmonary nodules in the specified group (Vector, ESPN and ESPN + sh-ZEB1). (E) Immunohistochemical analysis of ESPN, p-S6K and ZEB1 expression in osteosarcoma tissues. Data are expressed as the mean ± SD, *p < 0.05, **p < 0.01