Fig. 4
From: ESPN activates ZEB1-mediated EMT through the PI3K/AKT/mTOR axis to promote osteosarcoma metastasis
ESPN enhances EMT by promoting ZEB1 expression (A) Effects of ESPN knockdown on ZEB1, Snail, Slug and Twist protein expression as assessed by Western blot. (B) The expression levels of ZEB1, E-cadherin, N-cadherin, and Vimentin proteins were analyzed in different groups (sh-CON, sh-1 and sh-1 + ZEB1) of 143B and MNNG-HOS cells. (C, D) Scratch, transwell and invasion assays were performed to analyze the effects of knockdown of ESPN as well as simultaneous overexpression of ZEB1 on OS migration and invasion. (E) Effects of ESPN overexpression on ZEB1, Snail, Slug and Twist expression as assessed by Western blot. (F) Expression of ZEB1, E-cadherin, N-cadherin and Vimentin protein was analyzed upon overexpression of ESPN and concurrent knockdown of ZEB1. (G, H) To analyze the effects of knockdown of ZEB1 and simultaneous overexpression of ESPN on OS migration and invasion, we performed scratch, transwell and invasion assays. Scale bar = 50 μm