Fig. 3

ESPN accelerates EMT in OS cells (A, B) The effect of ESPN knockdown or overexpression on the expression of EMT-related marker protein in OS cell lines was examined using Western blot. (C, D) Immunofluorescence analysis of E-cadherin, N-cadherin, and Vimentin expression after treatment of 143B and MNNG-HOS cells with sh-CON or sh-1. DAPI is the blue color of nuclei. (E) Immunofluorescence analysis of E-cadherin, N-cadherin, and Vimentin expression after treatment of MG63 cells by Vector or ESPN. DAPI staining is shown in blue and indicates nuclei. (F, G). Immunofluorescence scoring of E-cadherin, N-cadherin and Vimentin. Data are expressed as the mean ± SD, scale bar = 20 μm, *p < 0.05, **p < 0.01