Fig. 2

ESPN promotes OS progression in vitro (A) Knockdown efficiency of ESPN in OS cells (MNNG-HOS and 143B) was detected by Western blot. (B) Scratch and (C) Transwell assays for cells migration and invasive ability. These data represent three independent experiments. (D) The results of CCK-8 experiments showed that ESPN knockdown inhibited the proliferative ability of MNNG-HOS and 143B cells. (E) Cells survival of OS cells before and after ESPN knockdown was detected by colony formation assay. (F) The overexpression efficiency of ESPN in OS cells (MG63) was detected by Western blot. (G, H) Scratch and (I) Transwell assays for cells migration and invasion ability. (J, K) The results of CCK-8 experiments and colony formation assay showed that ESPN gene overexpression increased the proliferation ability of MG63 cells. Data are expressed as the mean ± SD, scale bar = 50 μm, *p < 0.05, **p < 0.01 and ***p < 0.001