Fig. 1

Elevated ESPN expression in OS (A) Based on the Human Osteosarcoma Gene Expression Database (https://hgserver1.amc.nl/cgi-bin/R2/main.cgi), Kaplan‒Meier analysis of the overall survival in 88-patient OS group by ESPN expression and metastasis status. (B) Immunohistochemical methods (IHC) were applied to detect ESPN expression in OS and osteochondroma (OC) tissues. (C, D) The results were statistically analyzed according to the expression of ESPN in OS and OC. (E) Quantitative polymerase chain reaction was used to detect the expression of ESPN in normal osteoblast cells lines (hFOB1.19) and OS cells lines (U-2OS, MNNG-HOS, MG63 and 143B). (F) Western blot for ESPN expression in a normal osteoblast cells line (hFOB1.19) and OS cells lines (U-2OS, MNNG-HOS, MG63 and 143B). Values are expressed as mean ± standard deviation, scale bar = 100 μm, *p < 0.05, **p < 0.01 and ***p < 0.001