Fig. 6

In vitro experiments confirm that under high methionine or high Hcy environments, histone H3K79hcy abnormal modification occurs and regulates the disordered expression of FSSA genes: (A) EAHY926 cells were cultured in vitro with high methionine, high HCY, or high HTL treatments. Western Blot (WB) was used to detect the protein expression levels of MARS, TRX, GPX1, ICAM1, and the modification levels of H3K79hcy. (B) In cells treated with high methionine or high HCY, after knocking down MARS expression, protein expression levels of TRX, GPX1, and ICAM1 were detected by Western Blot. (C) Immunofluorescence technique was used to detect the expression levels and distribution of H3K79hcy in cells treated with high methionine or high HCY, or in cells where MARS expression was additionally knocked down. (D) ChIP-qPCR technique was used to detect the binding ability of H3K79hcy to FSSA pathway genes in cells of different treatment groups