Fig. 5

Elevated modification of H3K79hcy regulate FSSA pathway genes in aorta tissue of HHYP rats. (A) In HHcy treated mouse neural stem cells, H3K79hcy exerts an epigenetic regulatory effect on the genes Txn1/2, Gpx1, Ask1, Nfkb1/2, and Icam1. (B) WB analysis the modification level of H3K79hcy and expression level of MARS protein in rat aortic tissue, n = 6. (C) The enrichment levels of H3K79hcy in the promoters of TRX pathway genes Txn1/2, Gpx1, Ask1, Nfkb1/2, and Icam1, which were detected by ChIP-qPCR, n = 3. (D) RT-qPCR analysis of relative mRNA levels of TRX pathway genes in aorta tissues of the three rat groups (normalized to Gapdh), n = 3/group, two technical replicates were performed for each sample