Fig. 4

Decreased TRX, GPX1 and increased ICAM1 expression levels in aorta tissue of HHYP rat model. (A) Change of systolic blood pressure (SBP) from 8 to 16 weeks of intervention. (B) Hcy content in rat plasma, and folate content in serum (n = 12). (C) Typical diagram of TRX, GPX1 and ICAM1 protein expression and distribution in rat aorta tissues detected by IHC. Arrows indicate thickened aortic vessels in the HHYP group; Blue indicates the nucleus stained with hematoxylin; brown indicates the staining of target protein (Magnification: X200), n = 3, the IHC calculation results for each rat were derived from two fields of view. Bars: 50 μm. (D) Representative WB staining bands are shown (TRX, GPX1 and ICAM1). Equal amounts of loading were confirmed by Gapdh staining, n = 3/group