Fig. 1

TMT-6plex quantification results. (A) A schematic diagram of the sample grouping information and the TMT6 labeling quantitative process. (B) Homocysteine metabolic chart, where a diet low in folate or high in methionine can lead to elevated levels of Hcy, which can further be metabolized into homocysteine thiolactone (HTL) under the catalysis of MARS enzymes. The latter can cause homocysteinylation modifications of histones or proteins. (C) The plasma contents of Hcy, serum contents of folate, and vitamin B12/6. (D) The Venn diagram displays the number of proteins detected in three biological replicates of TMT6 labeling quantification. (E) Principal component analysis (PCA) analysis of the control and HHYP TMT6 quantification data globally. (F) Volcano plot shows the 1000 proteins in the plasma of patients, proteins with fold change > 1.3 difference in HHYP compared to controls is shown in blue (upregulated) and orange plots (upregulated). The horizontal dashed grey line represents the selected significance threshold. The vertical broken grey lines correspond to the upregulated and downregulated fold change thresholds. (G) Heatmap displays the protein expression intensity averaged across individuals