Fig. 2

Lenvatinib triggers ERS and further inducing ICD in HCC cells. A Western blotting was used to detect the effect of lenvatinib combined with an ERS inhibitor ISRIB (0.2 μM) on the expression of ATF4, CHOP, and p-PERK in Huh7 cells. B The gray value results of the western blotting experiment in Huh7 cells. C Flow cytometry demonstrated the effect of ISRIB combined with lenvatinib on CRT translocation to the membranes of HCC cells. D The HMGB1 levels in cell supernatant after treatments of ISRIB combined with lenvatinib in Huh7 cells. E The ATP level was analyzed after lenvatinib or ISRIB treatment in the cell supernatant of Huh7 cells. Each experiment is conducted in triplicate, and the represented data are standard deviation (SD) (±) mean for each experiment. *P < 0.05