Fig. 7
GSK461364 and Enz induce ferroptosis via SLC7A11 downregulation in PCa cells. A, B Lipid peroxidation assays were performed to assess ferroptosis induction in shNC and shPLK1 cells following treatment. C, D Lipid peroxidation assays were conducted to evaluate ferroptosis induction in wild-type PCa cells after treatment. E Ferro-orange staining for intracellular Fe2+ was performed in shNC and shPLK1 cells treated for 48 h with or without Enz (10 μM). Scale bar, 100 μm. F Ferro-orange staining for intracellular Fe2+ in wild-type PCa cells treated for 48 h with or without Enz (10 μM). Scale bar, 100 μm. G Western blot analysis showing SLC7A11 expression levels following treatment with or without Enz in shNC and shPLK1 cells. H Western blot analysis demonstrating SLC7A11 expression levels after treatment with GSK461364, Enz, or their combination. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (A–D). A–D Data are presented as mean ± SD. Representative data of triplicate experiments are shown. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant