Fig. 8

Identification of differentially expressed proteins by label-free proteomic analysis. (A) Flow diagram of proteomic analysis. (B) A volcano plot was utilized to depict the differential expressed proteins in HG-induced RMECs between TRIM44 knockdown and its negative control. The accession of the top 10 differentially expressed proteins was labeled in the diagram. (C) A heatmap with hierarchical clustering was utilized to depict the differential expressed proteins. A single row represented each differentially expressed protein, whereas a single column represented each cell sample. (D) KOG annotation of differentially expressed proteins. These proteins were classified into 19 functional clusters. LC-MS/MS, liquid chromatography-mass spectrometry; DEPs, differentially expression proteins; KOG, Eukaryotic Orthologous Groups; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes