Fig. 6

TRIM44 is transcriptionally regulated by KLF9. (A) Western blot assay was performed in RMECs treated with different conditions to assess the protein expression of KLF9. (B-C) The efficiency of lentiviral infection was verified by detecting KLF9 expression in untreated or treated RMECs. (D) The mRNA level of TRIM44 were detected in KLF9-overexpressed RMECs with HG treatment. (E-F) The protein expression levels of TRIM44 were detected in KLF9-overexpressed RMECs without or with HG stimulation. (G) Luciferase activity of TRIM44 promoter was measured in 293T cells co-transfected with KLF9 overexpression vector or emptor vector. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. n = 3 per group