Fig. 4

TRIM44 knockdown alleviates the migration and invasion of RMECs induced by high glucose. (A-B) Western blot assay was performed to verify the efficiency of lentiviral infection in untreated or treated RMECs cells. (C) Cell viability was assessed in RMECs after 72 h-infection. (D) EdU incorporation was detected via flow cytometry, and (G) the number of EdU-positive cells was quantified. (E-F) Cell migration (magnification × 100) and invasion (magnification × 200) ability were detected by wound healing assay and Transwell assay, respectively. The quantification of (H) the percentage of wound-healing rate and (I) the number of invasive cells were exhibited, respectively. EdU, ethynyl-2-deoxyuridine, ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. n = 3 per group