Fig. 2

Circulating and EVs-associated LGALS3BP could function as early detection biomarker of neuroblastoma lesions in a human neuroblastoma pseudometastatic model. (A) EVs-associated LGALS3BP levels detected in ELISA (pg/mL) per µg of EVs isolated from mouse serum collected 14 days after NSG mice (n = 6) were tail-vein injected with human neuroblastoma SKNAS cells. Tumor free mice (n = 2) were considered as controls. (B) Representative NTA profile of EVs isolated from mouse serum after 14 days from tail-vein injection of SKNAS cells. (C) Circulating LGALS3BP levels detected in ELISA (pg/mL) in mouse serum (diluted 1:4) after 14 days (n = 6). Tumor free mice (n = 2) were considered as controls. Number of metastases detected in (D) liver, (E) lung, and (F) kidney of NSG at 14 days post-injection