Fig. 1

Manufacturing of PB-derived CD5 CAR-NK cells with engineered K562 feeders. (a) Schematic diagram illustrating the CD5 CAR constructs with or without the HSV-TK suicide gene system (2504 and 2505, respectively). (b) Procedure for generating CD5 CAR-NK cells from PB. (c) Representative flow cytometry analysis showing the transduction efficiency of CAR-NK cells. (d) Variability in CAR transduction efficiency among NK cells from different donors and its temporal changes. (e) Expansion of CAR-NK cells ex vivo. (f) IL-15 secretion profile of CAR-NK cells. (g) Longitudinal analysis of the activating and inhibitory receptor profiles of CAR-NK cells from two healthy donors. The mean fluorescence intensity (MFI) of each marker was normalized using side scatter (SSC) values, and the square root of the resulting values was plotted as a heatmap