Fig. 4

(A) PCA plot representing all CD45 ROIs illustrated with the first three principal components. Three clusters are shown. (B) General heat map with unsupervised clustering performed based on CD45 ROIs. The color scale represents the log2 change from the geometric mean off all probes in the analysis. Annotations: Neg (UnLN); Pos (LNM); B-cell follicles with GC (Cluster 1); B-cell follicles without GC (Cluster 2); Interfollicular T-cell rich region (Cluster 3). (C) Quantification of bulk CD20⁺ B-cells in UnLN compared to LNM using QuPath software. Statistics were performed with paired students T-test. (D-F) Volcano plots showing the statistical significance versus the magnitude of change in protein expression between five paired LNM (left) versus UnLN (right). Linear mixed model (LMM) adjusted for matched donors was applied as statistical tests. Proteins with significant P-value < 0.05 are shown in blue, with proteins showing a log2 fold change above 0.5 plotted in red or green depending on the direction (D) B-cell follicles with GC (Cluster 1) associated proteins with significant difference in expression between paired LNM and UnLN. (E) B-cell follicles associated proteins (Cluster 1 and 2) with significant difference in expression between paired LNM and UnLN. (F) Interfollicular T-cell rich region (Cluster 3) associated proteins with significant difference in expression between paired LNM and UnLN