Fig. 1

(A-B) Representative of breast cancer lymph node tissue stained with H&E and IHC (CD169) (A) SCS CD169⁺ macrophages and (B) MS CD169⁺ macrophages of whole lymph node sections from LNM together with zoomed visualizations of areas (DAB and segmentation in QuPath) (C) Quantification of bulk CD169⁺ cells (D) SCS CD169⁺ macrophages (E) MS CD169⁺ macrophages in UnLN compared to LNM using QuPath Software. Error bars indicate SEM, Statistics were performed with paired students T-test, * P < 0.01, ** P < 0.05, *** P < 0.001. N = 5