Fig. 2

Treg cells produce high levels of AREG. (A) Spleen CD4⁺ T cells were isolated and cultured under neutral (Th0), Th17 and Treg-polarization conditions for 5 days, and Areg expression was determined by qRT-PCR. (B) Spleen WT and Areg^−/− CD4⁺ T cells were isolated and cultured under Treg conditions, Tregs ratio was detected by flow cytometry. (A) one-way ANOVA; (B) Unpaired Student’s t-test; ^***P < 0.001; ns: not significant