Fig. 6
From: CXCL12/CXCR4 axis mediates CD8 + T cell overactivation in the progression of viral myocarditis
CXCL12/CXCR4 axis promoted the cytotoxicity of CD8+T cells. (A) Volcano plot exhibiting the top five upregulated and downregulated genes of CD8+T cells in FM acute phase compared with control. Red refers to upregulated genes, blue refers to downregulated genes. (B) Violin plots showing the expression of CXCR4 in three groups. (C-D) The expression of CXCR4 in splenic CD8+T cells (C) and heart (D) of mice in MC group and control group tested by qRT-PCR. (n = 5) (E) The expression of CXCL12 in the heart of mice in two groups tested by qRT-PCR. (F) The expression of CXCL12 in HL-1 cells stimulated by CVB3 or not, tested by qRT-PCR. (G) The level of CXCL12 in the supernatant of HL-1 cells stimulated by CVB3 or not, tested by ELISA. (H) Immunofluorescence revealed the colocalization of CXCR4 (green) and CD8+T cells (red) in the myocardium of MC mice at day 7. Nuclei were stained with DAPI (blue). Scale bar, 275 μm. (I-M) The expression of effective factors GZMA(I), GZMB(J), Perforin (K) and IFN-γ (L) in CD8 + T cells stimulated with three different conditions in vitro. Statistic analysis is shown in M. (N-O) The apoptosis rate of HL-1 cells co-cultured with CD8 + T cells under three different conditions, or with PBS in vitro. (P) The level of CXCL12 in serum of FM patients and healthy controls tested by ELISA. Unpaired t-test was used for analysis. Data are presented as mean ± SD (n = 3 biologically independent samples). *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test)