Fig. 4

Depleting CD8⁺T cells in CVB3-induced viral myocarditis. (A) The clear efficiency of CD8⁺T cells confirmed by flow cytometry. (B) Schematic workflow of CD8⁺T cell deletion. (C) Weight changes of mice in different groups during the experimental process. (D) Representative echocardiographic image of different groups. (E-F) The ejection fraction (E) and fraction shortening (F) change of three groups. (G) Representative HE staining images in different groups. Scale bar, 20 μm. (H) Tunel staining revealed cell apoptosis in the myocardium in different groups. Broken DNA was labeled with CF488 (green). Nuclei were stained with DAPI (blue). Scale bar, 50 μm. (I-J) The plasma expression level of cardiac troponin T(cTnT) (I) and TNF-α (J) in different group. Unpaired t-test was used for analysis. Data are presented as mean ± SD (n = 5 biologically independent samples). *p < 0.05; **p < 0.01; ***p < 0.001