Fig. 3
From: CXCL12/CXCR4 axis mediates CD8 + T cell overactivation in the progression of viral myocarditis
The accumulation and cytotoxicity of CD8+T cells in CVB3-induced viral myocarditis mice. (A) The representative flow cytometry images of CD8+T cells in PBMCs of myocarditis (MC) mice and control (Con) mice at day 7. (B) The representative flow cytometry images of CD8+T cells in spleens of MC mice and control mice at day 7. (C) Immunofluorescence revealed the enrichment of CD8+T cells (red) in the myocardium of MC mice at day 7. Scale bar, 275 μm. (D-E) The histogram of proportion of CD8+T cells in PBMCs (D) and spleens (E) of MC mice and control mice at day 7. (F-J) The expression of cytotoxic genes GZMA(F), GZMB(G), Perforin(H), IFN-γ(I) and TNF-α(J) was significantly upregulated in splenic CD8+T cells of MC mice compared with that in control mice tested by qRT-PCR. Unpaired t-test was used for analysis. Data are presented as mean ± SD (n = 3 biologically independent samples). *p < 0.05; **p < 0.01; ***p < 0.001