Fig. 2

Analysis of T cells, TCRs and subtypes of CD8⁺T cells. (A) Identification of peripheral blood NK cells and T cells across all samples into seven subtypes, including five T cell subtypes, and two NK cell subtypes. (B) Fraction of the five T cell subtypes and two NK cell subtypes among three groups. (C-E) Cellular immunity data from clinical laboratory tests by flow cytometry (FCM) showed the percentage of CD4⁺T cells (C), CD8⁺T cells (D), and the radio of CD4⁺T/CD8⁺T cells (E), in children with acute FM (n = 29) and control children (n = 14). Unpaired t-test was used for analysis. *p < 0.05; **p < 0.01; ***p < 0.001 (F) Identification of peripheral blood CD8⁺T cells across all samples into five subtypes. (G) Percentages of each CD8⁺ T cell subtype in total CD8⁺ T cells among three groups. (H) Dot plot showing the expression of marker genes of each CD8⁺T cell type in each cluster. (I-L) Dot plot of DEGs among three groups in CD8⁺ Tn cells(I), CD8⁺ Tscm/cm(J), CD8⁺ Tem(K), CD8⁺ Teff cells(L). (M) UMAP embedding of all CD8⁺ T cell subtypes, overlaid with the RNA velocity stream. (N) Subclustered NK cells and T cells colored by clonotype sizes (Left) and cell types (Right). (O) TCR clones detected among NK cell and T cell subsets, colored by their clonotype sizes. (P) Subclustered CD8⁺ T cells colored by clonotype sizes (Left) and cell types (Right). (Q) The clonal-size percentage across three conditions. Clonotypes whose clone size > 1 were identified as clonally expanded clones.