Fig. 1

Analysis of peripheral blood mononuclear cell (PBMCs) populations in children with fulminant myocarditis. (A) Schematic of the experimental workflow. PBMCs were collected from healthy donors and children with fulminant myocarditis (FM) at the acute and recovery phases for single-cell RNA sequencing (scRNA-seq), single-cell T cell receptor sequencing (scTCR-seq), and single-cell B cell receptor sequencing (scBCR-seq). (B) Total PBMC clusters gained by the dimensional reduction using the uniform manifold approximation and projection (UMAP) across all samples. (C) Identification of PBMC clusters into four major immune cell types. (D) Dot plot showing the expression of marker genes of each cell type in each cluster. (E) Percentages of the four immune cell types among three groups. (F) Feature maps with major immunological canonical markers in UMAP. (G) Violin plots with major immunological canonical markers across all clusters. (H) Cellular immunity data from clinical laboratory tests by flow cytometry (FCM) showed the percentage of T cells (up), B cells (medium), and NK cells (down), in children with acute FM (n = 29) and control children (n = 14). Unpaired t-test was used for analysis. *p < 0.05; **p < 0.01; ***p < 0.001