Fig. 7

TMEM176B inhibited the proliferation and metastasis of OC cells via the Wnt/β-catenin signaling pathway. A2780 cells were transfected with negative control or TMEM176B overexpresion lentiviruses, and then incubated with LiCl (10 mmol/L) or vehicle (DMSO) for another 24 h. (A-C) Western blotting was used to determine the reversal efficiency of LiCl on the key proteins of the Wnt/β-catenin signaling pathway. (D-F) The impact of LiCl on the colony-forming ability. (G-H) The effect of LiCl on the migration was assessed by wound healing assays. The histogram showed the statistical analysis of the wound healing rate at 48 h. Scale bars, 500 μm. (I-J) The impact of LiCl on the metastasis was evaluated using transwell assay. Scale bars, 500 μm. *P < 0.05; **P < 0.01; ***P < 0.001, n = 3