Fig. 2

TMEM176B knockdown promoted OC cells proliferation and metastasis in vitro. (A) The protein expression levels of TMEM176B in various OC cell lines, with A549 as a positive control. (B) OVCAR3 cells were transfected with shNC or shTMEM176B lentiviruses. The knockdown efficiency was verified by Western blotting. (C) The effect of TMEM176B knockdown on the proliferation of OVCAR3 cell line. (D-F) The effect of TMEM176B knockdown on the colony-forming ability of OVCAR3 cells. (G-H) Wound healing assays were used to evaluate the ability of TMEM176B knockdown on cell migration. The histogram showed the statistical analysis of the wound healing rate at 48 h. Scale bars, 500 μm. (I-J) The effect of TMEM176B knockdown on the migration and invasion of OVCAR3 cells was assessed by transwell assay. Scale bars, 100 μm. (K-L) The effect of TMEM176B knockdown on the protein levels of EMT markers. **P < 0.01, n = 3