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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration

Fig. 3

Verification of the methylation level of the cg12414070 site in ccRCC and its regulatory effect on FBXO21 expression. A The methylation levels of cg12414070 in 54 ccRCC and 10 AN tissues were detected by pyrosequencing. B Comparison of the methylation level of the cg12414070 site between T1/T2 and T3/T4 tumors, G1/G2 and G3/G4 tumors, and M0 and M1 tumors. C The correlation between the methylation level of cg12414070 and the protein expression level of FBXO21. D Detection of FBXO21 protein expression in ccRCC cell lines by Western blot. E Detection of the methylation level of the cg12414070 site in ccRCC cell lines by pyrosequencing. F Detection of the methylation level of cg12414070 after treatment with demethylating drugs in ccRCC cell lines by pyrosequencing. G Detection of the protein expression of FBXO21 after treatment with demethylating drugs in ccRCC cell lines. AN: adjacent normal

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Journal of Translational Medicine

ISSN: 1479-5876

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