Fig. 7

Regulatory effects of DMC-GF on SLC25A1-mediated GSC proliferation. (A) Western blot analysis confirming overexpression of SLC25A1 (OE-SLC25A1) in GSCs (n = 3). (B) CCK-8 analysis of the proliferation rates of GSCs after SLC25A1 overexpression and subsequent treatment with DMC-GF (n = 5). (C) Flow cytometry assays of the apoptosis rates in GSCs after SLC25A1 overexpression and DMC-GF treatment (n = 3). (D) tumour growth in orthotopic Patient-Derived tumour Xenograft (PDX) models using PD-GSC2 with altered SLC25A1 expression (n = 4). Three independent biological replicates were included for (A) and (C). ∗∗, P<0.01