Fig. 6
From: Asymptomatic vivax malaria is associated with an IFN-γ-program on adaptive immunity
B cell signature suggests a TH1-biased commitment of B cell lineages due to increased AtM compartment. B cells from SY, ASY, and CTL were analyzed by flow cytometry analysis. (A) Representative dot-plot showing a t-SNE projection of B cell populations analyzed by group: Activated memory B cells (AcM, CD27+CD21−CD20+CD10−CD19+), Atypical memory B cells (AtM, CD27−CD21−CD20+CD10−CD19+), Immature B cells (CD10+CD19+), Naïve B cells (CD27−CD21+CD20+CD10−CD19+), Plasma cells (CD21−CD20−CD10−CD19+), Classical memory B cells (CM, CD27+CD21+CD20+CD10−CD19+). (B) Frequencies of Immature B cells, Plasma Cells, CM, AcM, AtM, and Naïve B cells in CD19+ B cells by group. Data is represented as scattered dot-plots over boxplots with medians and interquartile range. Each dot represents a single individual. Asterisks over connecting lines represent significant differences between groups by Dunn’s test. (C) Frequencies of Ki67-expressing cells among CM, AcM, AtM, and Naïve over total CD19+ B cells. (D) Spearman correlation between relative parasitemia (copies/µL) and the frequency of ki67+ atypical memory B cells from ASY. (E) Spearman correlation between antibody levels against PvAMA166, PvMSP119, and PvDBPIIbrz−2 and the frequency of Ki67+ atypical memory B cells from ASY. Spearman’s R and p-values are displayed on graphs. The line represents the trend line by a linear regression model