Fig. 5

Increased infiltration of exhausted T cells in the metastatic niche (MN). A UMAP plot of T/NK cells. B Marker expression for T/NK cells. C Proportion of T/NK cell subpopulations. D Differential volcano plots of various T/NK cell subpopulations comparing MN with control. E GO enrichment bubble plot showing the results of GO pathway enrichment for differential genes between MN exhausted T cells and control exhausted T cells. F Bar chart showing the correlation between the proportion of N1 neutrophils and exhausted T cells in colorectal cancer liver metastasis patients. G Bar chart showing the correlation between the proportion of Kupffer2 macrophages and exhausted T cells in colorectal cancer liver metastasis patients. H Bar chart showing the correlation between the proportion of N1 neutrophils and Kupffer2 macrophages. I Bar chart showing the correlation between the proportion of N1 neutrophils and MAC2 macrophages. The p-values in these figures were calculated using the Wilcoxon test