Fig. 4

Increased infiltration of suppressive macrophages in the metastatic niche (MN). A UMAP plot of macrophages. B Bubble plot of Kupffer cell markers. C Display of the top 5 markers for each macrophage cluster. D Proportion of macrophage clusters. E UMAP trajectory plot of macrophages in pseudotime. F Heatmap showing the expression of genes in Kupffer2 and Kupffer3 cells, with the top 40 genes upregulated in Kupffer2 compared to Kupffer3. G GO enrichment bubble plot of differential genes between Kupffer2 and Kupffer3. GO enrichment was performed with p < 0.05 and LogFC > 1, showing the top 10 functions based on p-value. H KEGG pathway enrichment bubble plot. I UMAP plot of CD14 signature expression values. J Diagonal plot of differential genes between MAC2 and MAC1, percentage difference is calculated by subtracting the expression levels between two cell types. K, L UMAP plots of M1 and M2 macrophage signature expression scores