Fig. 3

N1 neutrophils infiltrate more in the liver pre-metastatic niche (PMN) and metastatic niche (MN) are associated with poor prognosis in colorectal cancer liver metastasis patients. A UMAP plot of myeloid cells. B Myeloid cell marker expression plot. C Proportion of myeloid cell subpopulations in control, PMN, and MN. D UMAP plot of neutrophils. E Proportions of neutrophil subpopulations. F Proportion of N0-N3 neutrophils in the PMN liver of the colorectal cancer liver metastasis model. G Proportion of N0-N3 neutrophils in the PMN liver and MN of the pancreatic cancer liver metastasis model. H Prok2⁺ neutrophils in the livers of the control, PMN, and MN groups through co-staining Mpo and Prok2 using immunofluorescence (IF). I Proportion of N0-N3 neutrophils across different pseudotime stages. J Proportion of cells at different pseudotimes in control, PMN, and MN. K Heatmap showing neutrophil-related signatures in N0-N3 neutrophils, with values scaled from −1 to 1. L Survival curve of colorectal cancer liver metastasis patients based on the high or low proportion of N1 neutrophils. M Expression levels of the N1 neutrophil signature in peripheral blood and liver tissues of colorectal cancer liver metastasis patients and healthy controls. "Liver normal" and "liver tumor" refer to liver tissues from healthy donors and colorectal cancer liver metastasis patients, respectively. "PBMC normal" and "PBMC tumor" refer to peripheral blood mononuclear cells from healthy donors and colorectal cancer liver metastasis patients, respectively