Fig. 2
The differential genes and pathways in hepatocytes between the pre-metastatic niche (PMN) and metastatic niche (MN). A Differential volcano plot of hepatocytes between the PMN group and the control group. B Differential volcano plot of hepatocytes between the MN group and the control group. C Differential volcano plot of hepatocytes between the MN group and the PMN group. Red dots represent the top 20 upregulated genes, orange dots represent upregulated genes with p < 0.05 and Log2fc > 1, and blue dots represent downregulated genes with p < 0.05 and Log2fc < −1. GSEA analysis of differential pathways in hepatocytes between the PMN group vs control (D), MN group vs control (E), and MN group vs PMN group (F). Red indicates upregulated pathways, and blue indicates downregulated pathways. NES represents normalized enrichment score. G Differential volcano plot in the pre-metastatic niche (PMN) liver of the pancreatic cancer orthotopic liver metastasis model compared to the control group. H Differential volcano plot in the PMN liver of the pancreatic cancer spleen metastasis model compared to the control liver. I Differential volcano plot in the PMN liver of the colorectal cancer orthotopic liver metastasis model compared to the control liver, showing the top 19 upregulated genes in PMN compared to control