Fig. 1

Single-cell atlas of liver in a mouse model of colorectal cancer liver metastasis. Flow cytometry analysis of GFP signals in liver tissues from control (A), pre-metastatic niche (PMN) (B), and metastatic niche (MN) (C) groups of the colorectal cancer orthotopic mouse model. D Diagram of liver single-cell suspension preparation. E Uniform Manifold Approximation and Projection (UMAP) plot of liver samples from all groups. F Cell type annotation markers. G The proportion of each cell type in different groups. H The proportion of tumor cells in the control, PMN, and MN groups. The proportion of T/NK cells (I), Myeloid cells (J) and B cells (K) among immune cells in the control and PMN groups. P value was calculated using Wilcoxon test